Molecular Assessment of C4d-Positive Renal Transplant Biopsies Without Evidence of Rejection

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C4d positivity is often associated with acute cellular rejection in renal transplant biopsies following Campath-1H (Alemtuzumab) induction.

Peritubular capillary C4d positivity, a marker for antibody-mediated rejection, is observed in approximately 20-50% of indicated renal transplant biopsies and in just 2% of unremarkable protocol biopsies. However, C4d staining has not been evaluated in protocol renal biopsies from patients with Campath-1H induction treatment, and the association between various types of inflammatory cells and a...

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The Importance of C4d in Biopsies of Kidney Transplant Recipients

Antibody-mediated rejection (AMR) is highly detrimental to the prolonged survival of transplanted kidneys. C4d has been regarded as a footprint of AMR tissue damage, and the introduction of C4d staining in daily clinical practice aroused an ever-increasing interest in the role of antibody-mediated mechanisms in allograft rejection. Despite the general acceptance of the usefulness of C4d in the ...

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C4d staining in renal allograft biopsies with early acute rejection and subsequent clinical outcome.

BACKGROUND AND OBJECTIVES Diffuse C4d staining in peritubular capillaries (PTCs) during an acute rejection episode (ARE) is the footprint of antibody-mediated rejection. In current clinical practice, diffuse C4d+ staining during acute rejection is regarded as an inferior prognostic sign. This case-control study investigated the prognostic role of mere C4d staining for graft outcome during an AR...

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Comparison of C4d immunostaining methods in renal allograft biopsies.

Immunostaining of renal allograft biopsies for C4d deposition has become an important diagnostic tool in the recognition of humoral-mediated graft rejection. The majority of studies have been performed on frozen tissue sections with one of several commercially available antibody reagents. However, only a single small series that compared reagents or methods, including staining of formalin-fixed...

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Renal Transplant Rejection

Introduction: Determining if the cause of renal allograft dysfunction in the early post transplant period is due to rejection or other reasons is a daunting task for the clinician. Clinical signs and symptoms alone are not helpful in clearly distinguishing the numerous causes of renal dysfunction. Various noninvasive tests including imaging studies have also been unsuccessful in distinguishing ...

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ژورنال

عنوان ژورنال: Kidney International Reports

سال: 2019

ISSN: 2468-0249

DOI: 10.1016/j.ekir.2018.09.005